Antibiotic resistance has emerged as a major threat to public health. In Gram-negative bacteria, efflux complexes consist of an inner-membrane pump, a periplasmic adaptor protein and an outer-membrane channel. Drug efflux protein complexes confer multidrug resistance on bacteria by transporting a wide spectrum of structurally diverse antibiotics. A new molecular challenge in combat to this transport is the search for new molecules to block efflux and restore drug susceptibility to resistant clinical strains. In the first step, the outer and inner membrane of efflux pumps sequences were aligned. In the following, 3D flexible alignment was performed to identify similar conformational...