REAL Space

The World's Largest Make-on-Demand Chemical Space for Drug Discovery

Enamine's REAL Space (REadily AccessibLe) is the world's largest make-on-demand compound collection for early-stage drug discovery. Built from Enamine's in-house parallel chemistry expertise, it provides a combinatorially generated universe of drug-like molecules that can be synthesized, ordered, and delivered to your lab. Synthesis is completed in 3–4 weeks with an average success rate of over 80%.

The current release features more than 113 billion drug-like structures that can be searched and ordered directly. Every retrieved compound is ready to order from Enamine and delivered to your lab.

In this new update, REAL Space crosses the 100 billion mark, adding over 19 billion virtual product molecules and 10,555 building blocks. The collection is now built from 211,806 building blocks and 161 chemical reactions.

  • Make-on-demand: synthesized to order and delivered to your lab
  • Drug-like physicochemical properties (Ro5 and Veber compliant)
  • Rapid synthesis and delivery: 3–4 weeks, over 80% success rate
  • Constantly growing: regular updates with new reactions and building blocks
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REAL Space

Pioneering Vendor Catalog:
Years of Collaboration for a Lasting Impact

Since its joint launch by Enamine and BioSolveIT in February 2018, REAL Space has established itself as a widely used resource for drug discovery. Years of continuous development and regular updates have expanded its chemical diversity, reflecting both the success of the concept and its lasting relevance to the scientific community.

As our longest-running Chemical Space collaboration, REAL Space demonstrates how a thoughtfully designed compound collection can resonate with researchers. Its enduring appeal rests on a clear foundation: relevant chemistry, reliable synthetic accessibility, and efficient tools to identify compounds worth testing.
  • Established through years of use: A long-standing resource connecting Chemical Space exploration with experimental drug discovery.
  • Continuously growing and evolving: Regular updates introduce new building blocks and reaction protocols, expanding the opportunities available to researchers.
  • Designed for lasting scientific value: REAL Space brings chemical diversity and practical accessibility together, supporting compound discovery and follow-up across medicinal chemistry projects.

The Now 113 Billion Update: A New Order of Magnitude

The latest version of REAL Space is built on a continuously refined foundation of Enamine's in-house synthesis expertise. All results found using BioSolveIT's tools are ready to order directly from Enamine, with fast and reliable delivery to your lab.

161
Curated parallel synthesis reactions
211,806
In-stock reagents and building blocks
113.7 B
Virtual products, searchable and orderable

What Makes REAL Space Unique?

REAL Space is built entirely from Enamine's validated parallel synthesis protocols applied to in-stock building blocks. Every virtual product reflects chemistry that Enamine can perform at scale, ensuring a direct path from in silico hit to orderable compound.

  • Synthetically validated chemistry: Every compound is the product of a validated protocol applied to in-stock reagents at Enamine. The space reflects real, performable chemistry, not speculative enumeration.
  • Make-on-demand with reliable delivery: Compounds are synthesized to order within 3–4 weeks at a success rate exceeding 80%. Hits found in silico translate directly into physically accessible matter.
  • Beyond availability bias: REAL Space covers a broad, IP-free chemical universe, giving searches access to novel scaffolds and analogs not represented in standard in-stock collections.
  • Efficient on standard hardware: Despite containing over 113 billion structures, the REAL Space file is a few hundred megabytes. Searches run on a standard workstation in 2–4 minutes on average.
  • Two access modes: Available as an enumerated dataset and as a synthon-based combinatorial space, where results are generated on the fly to match the query.

The Evolution of REAL Space

Since its joint launch by Enamine and BioSolveIT in February 2018, REAL Space has grown from approximately 650 million to 113.7 billion virtual product molecules — around 175 times its initial size. The October 2026 update takes the collection beyond the 100 billion mark, continuing years of development with BioSolveIT's Pioneering Partner, Enamine.

The latest release combines 211,806 building blocks through 161 chemical reactions, implemented as 910 technical reactions. The table below traces the growth of REAL Space alongside selected REAL compound subsets available through ZINC.

Release Compounds Scale
ZINC15 120 M
2018-Q1 650 M
2018-Q2 3.8 B
2018-Q4 3.7 B
2019-Q1 5.1 B
2019-Q2 11 B
2019-Q4 13 B
ZINC20 1.8 B
2020-Q2 14 B
2020-Q4 16 B
2021-Q1 18 B
2021-Q2 19 B
2021-Q3 20 B
2021-Q4 21 B
ZINC22 37 B
2022-Q1 23 B
2022-Q2 29 B
2022-Q3 32 B
2022-Q4 34 B
2023-Q1 36 B
2023-Q2 36 B
2023-Q3 44 B
2023-Q4 48 B
2024-Q1 48 B
2024-Q3 70 B
2025-Q1 76 B
2025-Q3 83 B
2026-Q2 95 B
2026-Q4 ★ 113.7 B
Access with infiniSee

Access with infiniSee

REAL Space can be conveniently explored using BioSolveIT's Chemical Space navigation platform, infiniSee. Over 113 billion entries can be searched for relevant chemistry within seconds to minutes on standard hardware. Various search algorithms are available to identify the best matches for the objectives of a drug discovery project.

  • Scaffold Hopper (FTrees): Retrieves compounds based on fuzzy pharmacophore similarity.
  • Analog Hunter (SpaceLight): Screens for close analogs based on Tanimoto fingerprint similarity.
  • Motif Matcher (SpaceMACS): Mines for molecules containing an exact substructure or those with the highest maximum common substructure to the query.

Of course, REAL Space can also be accessed using the corresponding command-line versions of the modes (FTrees, SpaceLight, SpaceMACS).

Check out infiniSee

Want to Take It into the 3D World?

Chemical Space Docking® also enables structure-based screening of REAL Space within your target of interest. Executed within our drug design dashboard SeeSAR, molecules forming the most favorable interactions in the binding site can be efficiently mined on standard hardware, and without a connection to the internet.

This allows in-house projects to be streamlined without the involvement of third parties; you will work in a user-friendly environment with full control over your results.

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